Fig. 4.

ADCVI with wt b12, b12 variants, and DEN3 as an IgG1 control antibody. Human CD4 cells were infected with HIV-1 JR-FL (MOI of 0.1). Forty-eight hours postinfection, cells were washed and incubated with serial dilutions of antibodies (wt b12, b12 variants, and DEN3) and freshly isolated autologous PBMCs. Viral inhibition was measured by analyzing supernatant in a p24-specific ELISA 9 days postinfection. Increased or decreased affinity for FcγRIIa and FcγRIIIa results in a corresponding increase or decrease in viral inhibition, respectively. FcγRIIa up-variants are shown in blue, FcγRIIa down-variants are shown in pink, FcγRIIIa up-variants are shown in purple, and FcγRIIIa down-variants are shown in yellow. Values are means of triplicate wells. The assay was performed twice with similar results.