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Binding affinities for b12 variants to FcγRs determined by SPR
| Group and variant | FcγRIIa |
FcγRIIIa |
||
|---|---|---|---|---|
| KDa (μM) | Foldb | KDa (μM) | Foldb | |
| Wild-type b12 | 0.735 | 1 | 0.200 | 1 |
| FcγRIIIa up-variants | ||||
| I332E | 0.220 | 3.3 | 0.038 | 5.3 |
| SD/IE | 0.147 | 5.0 | 0.005 | 40 |
| SD/IE/AL | 0.246 | 3.0 | 0.002 | 100 |
| FcγRIIa up-variants | ||||
| G236A | 0.125 | 5.9 | 0.180 | 1.1 |
| SD/IE/GA | 0.036 | 20 | 0.011 | 18 |
KDs were obtained from global fits of Biacore sensorgrams (n=2).
Fold is relative to wt b12 (KD of b12/KD of variant).
