Fig. 7.

A short gene segment containing overlapping tat and rev and adjacent vpu sequences was transferred from the input X4 SHIVDH12 into R5 SHIVAD8 by intergenomic recombination. SGA and direct sequencing of plasma samples collected at weeks 31 and 64 p.i. were used to generate amplicons containing the first exons of the tat, rev, and vpu genes shown. The black bars below each sequence indicate the region contributed by the starting X4-tropic SHIVDH12. The numbers in parentheses denote the amplicons with the indicated sequence.