Fig. 11.

EMCV1.26Δ2A-infected cells die by apoptosis even under inhibition of cap-dependent translation. (a) BHK-21 cells were treated with a 100 nM inhibitor of cap-dependent translation, rapamycin (Rapa), for 24 h before infection at an MOI of 10 with EMCV1.26Δ2A. Cells were fixed at 14 hpi and immunostained with anti-EMCV VP1 followed by Alexa 546-labeled anti-mouse IgG (VP1), with anti-cleaved caspase 3 followed by Alexa 488-labeled anti-rabbit IgG (Cleav. Casp3) and with DAPI. (b) The graph represents the inhibition of cap-dependent translation during rapamycin treatment. BHK-21 cells were treated or not with 100 nM rapamycin for 24 h before transfection of a reporter plasmid of the cap-dependent translation (pRSV-βGal). The β-galactosidase activity was measured. Untransfected cells treated or not with rapamycin were used as negative controls.