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. 2011 Oct;85(20):10741–10754. doi: 10.1128/JVI.00394-11

Fig. 9.

Fig. 9.

EMCV1.26Δ2A-infected cells die by apoptosis. (a) BHK-21 cells were mock infected, treated with STS for apoptosis induction, or infected at an MOI of 10 with EMCV1.26 or EMCV1.26Δ2A. Cells were fixed at the indicated time postinfection and immunostained with anti-EMCV VP1 followed by PE-labeled anti-mouse IgG (red), with anti-cleaved caspase 3 followed by Alexa 488-labeled anti-rabbit IgG (green), and with DAPI (blue). (b) Dot plots obtained by flow cytometry of BHK-21 cells that were untreated (A), treated with STS (B), and infected at an MOI of 5 with EMCV1.26 for 8 h (C) or 14 h (D) or with EMCV1.26Δ2A for 8 h (E) or 14 h (F). Cleaved caspase 3 (apoptosis) and EMCV VP1 (viral infection) are graphed on x and y axes, respectively. (c) Percentage of apoptotic cells among infected BHK-21 cells. EMCV1.26 (black); EMCV1.26Δ2A (gray).