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. 2011 Oct;85(20):10669–10681. doi: 10.1128/JVI.05249-11

Fig. 10.

Fig. 10.

ZP6248.wt inefficiently utilizes CCR5. (A) ZP6248.wt and ZP6248.E312G pseudovirions were used to infect affinofile cells which express CD4 and CCR5 with independent induction systems. ZP6248.wt requires high CCR5 expression for infection compared to ZP6248.E312G, which can utilize very low levels of CCR5. Both Envs require moderate CD4 expression for infection. (B) The sensitivity to changes in CD4 and CCR5 levels was quantified by viral entry receptor sensitivity analysis (VERSA), which yields a single vector angle. Lower vector angles correspond to Envs that utilize CCR5 very efficiently as seen with the maraviroc-resistant Env MVC R3 (1°), while higher vector angles are associated with inefficient CCR5 use as seen with the V3-loop truncated virus TA1 (71°). ZP6248.wt has a vector angle of 44° compared to 0° for ZP6248.E312G. In addition, the vector magnitude can be quantified to assess overall infectivity. ZP6248.wt is 5-fold less infectious than ZP6248.E312G and 40-fold less infectious than MVC R3, consistent with other cell lines and primary cell data.