Fig. 3.

Infectivity of pseudoviruses containing ZP6248 Env glycoproteins. Viral pseudotypes bearing the indicated Env glycoproteins were used to infect TZM-bl cells stably expressing CD4, CXCR4, and CCR5. Infectivity was determined by measuring relative light units (RLU) in cell lysates 48 h after infection. ZP6248.wt represents the T/F Env, while ZP6248.E312G contained a single amino acid substitution in the V3 crown. A subtype C Env (ZM53 [32]) was used as a positive control, while Env-deficient pseudotypes (SG3Δenv) were used as a negative control.