Table 1.
Determination of coreceptor usage of ZP6248 in NP-2 cell linesa
| Env | Usage (RLU) of coreceptor |
||||
|---|---|---|---|---|---|
| GPR15 | APJ | FPRL-1 | CCR5 | CD4 only | |
| ZP6248.wt | 10,991 | 9,972 | 355 | 1,183 | 11 |
| ZP6248.E312G | 1,331 | 6,428 | 2,300 | 45,542 | 6 |
| NL4-3 (X4) | 82 | 9,433 | 6 | 220 | 23 |
| YU2 (R5) | 101 | 940 | 2,256 | 342,801 | 7 |
| NL4-3 without Env | 7 | 6 | 8 | 13 | 8 |
a
NP-2 cells expressing CD4 and the various potential coreceptors as noted were infected with NL4-3 luc+ pseudovirions. The ability to use the coreceptor was assessed by measuring relative light units (RLUs) in cell lysates and comparing the results to those for CXCR4- and CCR5-using control Envs.