Fig. 4.

Effect of 5ptase on virus release from QT6 cells. QT6 cells were cotransfected with DNAs encoding either HIV-1 Gag-GFP or RSV Gag-GFP plus a control plasmid (indicated by a minus sign) or a plasmid encoding myc-tagged 5ptase (indicated by a plus sign). Twenty-four hours posttransfection, virus-like particles (VLPs) were collected by centrifugation from the medium and the cells were lysed. The amounts of Gag-GFP in the lysate and in the VLP fractions were measured by Western blot analysis. Virus release was calculated as the amount of extracellular Gag as a fraction of total Gag. Error bars represent standard deviations from the mean from three independent experiments.