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Polymerase activities. Luciferase reporter activities in lysates of human 293T or avian DF-1 cells were determined after cotransfection of PB2, PB1, PA, and NP plasmids and a firefly luciferase minigene plasmid containing either an avian or human promoter (pPolI-Luc-RT or pPRC-PolI-Luc-RT, respectively) (five independent experiments). R65 PB2-627K (black bars) was set to 100% in comparison to the mutated PB2K627E (white bars).
