Table 6.
Analysis of QNE expression in mixed Env trimers expressing partial forms of the epitopesa
| Trimer(s) | Strain | % cells stained with indicated RhMAb |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2G12 | 2909 | 1.6F | 2.8F | 3.10E | 2.2G | 2.5B | 2.3E | LV23 | LW26 | ||
| wt | SF162 | 53.1 | 26.7 | 31.6 | 31.4 | 28.7 | 26.7 | 31.4 | 25.0 | 30.0 | 29.5 |
| Env 1 | SF162 (Z205F V1V2) | 38.2 | 0.0 | 0.1 | 0.1 | 0.1 | 0.0 | 0.0 | 0.0 | 0.1 | 0.0 |
| Env 2 | SF162 (CI A/E V3) | 51.9 | 0.1 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.1 | 0.0 | 0.1 |
| Env 1 + Env 2 | 56.8 | 0.0 | 0.2 | 0.1 | 0.0 | 0.0 | 0.1 | 0.0 | 0.0 | 0.2 | |
| Env 3 | SF162 (K160N/V161I) | 39.4 | 0.1 | 0.1 | 0.1 | 0.0 | 0.0 | 0.1 | 0.0 | 0.0 | 0.1 |
| Env 4 | SF162 (R315Q) | 38.0 | 9.8 | 9.0 | 9.1 | 4.7 | 0.1 | 0.0 | 1.6 | 0.2 | 3.8 |
| Env 3 + Env 4 | 37.7 | 2.0 | 5.5 | 2.9 | 0.4 | 0.7 | 1.0 | 2.0 | 0.0 | 0.2 | |
a
All MAbs were used at 5 μg/ml. Env plasmids were transfected at 1:1 ratio.