Fig. 2.

Phylogenetic analysis of the hexon gene. The hexon gene sequences of the novel AdVs and of published NHP AdVs and human HAdVs were aligned and subjected to phylogenetic analysis. The tree was reconstructed using maximum likelihood with the GTR+I+G model of substitutions, as described in Materials and Methods. Human, chimpanzee, bonobo, and gorilla AdVs are depicted in black, red, green, and blue, respectively. AdVs from wild and captive NHPs discovered in this study are marked with red and black dots, respectively. All other NHP AdVs were reported previously and originated from captive animals. The branches of all AdVs that could be allocated to one of the species Human adenovirus A to G or to the species Simian adenovirus A are colored separately. Branches left without extra coloring belong to AdVs for which tentative allocation to an AdV species was not possible. Bootstrap values are depicted at the nodes of the tree; nonsignificant values or those close to branch tips were omitted.