Fig. 8.

Tip Vps35-binding mutant viruses can transform T cells in the absence of IL-2. (A) Schematic diagram of cosmid-based recombinant virus generation and PCR analysis of recombinant viruses used in transformation assays. HVS wt C488 (parental virus) was used as a positive control. Recombinant viruses were generated to encode wt Tip or Tip 3Δ24 or Tip E/Q mutant. PCR amplification of cosmid overlapping regions (ORF3, ORF75, and StpC/Tip) were recombined to produce full-length virus. (B) Micrographs of aggregated, transformed T cells in cultures lacking IL-2. Recombinant wt virus is denoted (R).