Fig. 10.

Western blot detection of HIV-1 gp120-specific antibodies in sera of guinea pigs. The guinea pigs were immunized with rNDVs by the different routes described for Fig. 7. Purified BaL.1 gp120 was subjected to 10% SDS-PAGE under reducing conditions and transferred to a nitrocellulose membrane. The membrane was cut into strips and incubated with a 1:200 or 1:300 dilution of day 42 serum from each of the individual guinea pigs (GP) in each group. The position of the HIV-1 gp120 protein is indicated by the arrow in the left margin.