Fig. 4.

Incorporation of HIV-1 gp160 into recombinant NDV virions. Nine-day-old embryonated SPF chicken eggs were infected with either rLaSota or rLaSota/gp160 virus. The allantoic fluid of infected eggs was harvested 48 h postinfection and clarified by low-speed centrifugation, and NDV virions were purified by sucrose gradient centrifugation. The purified virus particles were subjected to 10% SDS-PAGE under denaturing and reducing conditions and subjected to Western blot analysis using a pool of gp120-specific monoclonal antibodies. The positions of the HIV gp160 precursor and gp120 proteins are indicated by arrows in the right margin. Molecular masses of the marker proteins (in kilodaltons) are shown in the left margin.