Fig. 6.

Release of wild-type HIV virions is impaired by IFN-induced BST-2. (A) Activated PBMCs were infected with R5-tropic HIV-1 for 4 h followed by removal of the virus and stimulation with IFN-α as indicated. On day 1 and 2 after infection, total PBMCs and CD4⁺ T cells were analyzed by flow cytometry for BST-2 surface expression (mean fluorescence intensity [MFI]). (B) In parallel, virus release was monitored by detection of secreted p24 capsid antigen by ELISA after centrifugation of culture supernatants through sucrose cushions. Shown are mean values ± SD of duplicates from one representative donor out of three.