Fig. 3.

POLST inhibits differentiation of 3T3-L1 preadipocytes and C2C12 myoblasts. (A) After retroviral infection 3T3-L1 cell pools were isolated that expressed GFP, POLST, W157S POLST, or SVST. Subsequently, cells were allowed to differentiate. At 7 days postinduction, differentiating cells were fixed and stained with Oil Red O. (B) After retroviral infection C2C12 myoblast cell pools were isolated that expressed GFP, POLST, PP2A⁻ W157S POLST, or SVST. Cell lysates were collected at 6 days postinduction of differentiation. Expression of MHC, a differentiation marker, was determined by Western blotting. PN116 antibody was used to detect the expression of POLST, while PAB419 antibody was used to detect the expression of SVST. α-Actin was used as a loading control.