Fig. 5.

Single-channel properties of arginine mutants. A, representative single channel events. Recordings were filtered at 2 kHz for analysis and 1 kHz for display. B, open dwell time distributions across patches were fit with the sum of three to four exponentials (time constants and relative areas are labeled). The first three time constants were not different in the mutants, but α1R132A receptors exhibited a small number of additional long-lived openings not seen for the other three constructs. C, summary of the first three open dwell time constants and relative areas. *, different by one-way ANOVA with post hoc Tukey's test at p < 0.05.