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. 1984 Apr 25;12(8):3427–3434. doi: 10.1093/nar/12.8.3427

Nucleotide sequence of cucumber pale fruit viroid: homology to hop stunt viroid.

T Sano, I Uyeda, E Shikata, T Ohno, Y Okada
PMCID: PMC318759  PMID: 6328420

Abstract

Double stranded cDNA of cucumber pale fruit viroid ( CPFV ) has been cloned by the method of Okayama and Berg (Mol.Cell.Biol.2,161-170 (1982] and the complete nucleotide sequence was established. The covalently closed circular molecules of single-stranded CPFV RNA consists of 303 nucleotides. The nucleotide sequence of CPFV was compared with the previously established sequence of hop stunt viroid (HSV), which consists of 297 nucleotides ( Ohno et al. Nucleic Acid Res.11,6185-6197 (1983]. CPFV differs from HSV in the nucleotide sequence at 16 positions which include 8 exchanges, 7 insertions and 1 deletion. Both viroids share about 95% sequence homology. Considering the pathogenic properties of both viroids together, it is concluded that CPFV is a cucumber isolate of HSV.

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Selected References

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