Figure 1.

FIP5 is enriched at the apical pole of polarized epithelial cells. (A–F) MDCK cells were grown in 2D (A–C) or 3D (D–F) cultures. Cells were fixed with 4% paraformaldehyde and stained with anti-FIP5 (A–F, green), anti-Occludin (A–C, red) or anti–e-Cadherin (D–F, red) antibodies. In A–C, images were taken at the apical, tight junction and basolateral levels of polarized MDCK cells. (G and H) Rat kidney sections were stained with anti-FIP5 antibody. In H, antibody was preincubated with a 20-fold excess of purified recombinant FIP5. Bars: (A–F) 8 µm; (G and H) 10 µm.