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. 2011 Oct 3;195(1):71–86. doi: 10.1083/jcb.201011112

Figure 2.

Figure 2.

FIP5 is required for the formation of a single lumen in 3D epithelial cysts. (A–E) MDCK-shFIP5 cells were grown for 9 d in the presence (B and D) or absence (A and C) of 1 µg/ml of dox. Cells were then fixed with 4% paraformaldehyde, stained with Hoechst DNA stain, and imaged by bright-field (A and B) or fluorescence (C and D) microscopy. E shows the quantitation of epithelial cysts with single lumen. Data shown are the means and standard deviations derived from three independent experiments (error bars). n is the total number of cysts analyzed. Insets show dox+ cyst expressing FIP5-GFP. (F–I) MDCK-shFIP5 cells were grown for 9 d in the presence (G and I) or absence (F and H) of 1 µg/ml of dox. Cells were then fixed with 4% paraformaldehyde and stained with anti-gp135 (F and G) or anti-cingulin (H and I) antibodies. Bars: (A and B) 40 µm; (C and D) 8 µm; (E) 16 µm (F–I) 8 µm.