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. 2011 Oct 3;195(1):71–86. doi: 10.1083/jcb.201011112

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© 2011 Willenborg et al.

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Figure 5. — SNX18 is FIP5-binding protein. (A) FIP5 was immunopreciptated from HeLa cell lysates with anti-FIP5 antibody. The immunoprecipitate was then separated by SDS-PAGE and stained with Coomassie dye. Proteins listed in the figure were identified by at least two peptides from the anti-FIP5 immunoprecipitate, and were not present in the IgG control. (B) Human SNX18 sequence. Boxed regions indicate the peptides identified in proteomic analysis of the immunoprecipitate from A. (C) SNX18 or FIP5 were immunoprecipitated from MDCK cell lysates and immunoblotted with anti-SNX18, anti-FIP5, and anti-SNX9 antibodies. (D) HeLa cells were cotransfected with myc-SNX18 and FIP5-GFP. Cells were lysed, and myc-SNX18 was immunoprecipitated with anti-myc antibodies and blotted with anti-SNX18 and anti-GFP antibodies. (E) Schematic representation of the domains present in SNX9 and SNX18 proteins. Numbers between the SNX9 and SNX18 schematics indicate the percentage of homology between the corresponding domains of these proteins.