Table II.
Collisional quenching of integration intermediates with three nascent chain TMSsa
| PMPb | TMSC-PMP separationc | nd | −MLT Ksve | +MLT Ksve | ΔKsv |
| M−1 ± SD | M−1 ± SD | M−1 | |||
| 3TML12,18K3159 | 3 | 4 | 1.9 ± 0.2 | 4.2 ± 0.3 | 2.3 ± 0.4 |
| 3TML12,18K3162 | 6 | 3 | 4.1 ± 0.2 | 4.1 ± 0.1 | 0.0 ± 0.2 |
| 3TML12,18K3167 | 11 | 3 | 4.2 ± 0.3 | 4.4 ± 0.2 | 0.2 ± 0.4 |
| 3TML12,68K3209 | 3 | 3 | 2.0 ± 0.2 | 4.1 ± 0.1 | 2.1 ± 0.2 |
| 3TML12,68K3212 | 6 | 3 | 3.9 ± 0.1 | 4.1 ± 0.2 | 0.2 ± 0.2 |
a
RTC preparation and spectroscopic analyses are described in Materials and methods.
b
PMP topogenic sequences and NBD locations are shown in Fig. 3 B.
c
TMSC, C-terminal end of TMS nearest the PTC; TMSC–PTC separation, nascent chain residues between TMSC and the PTC.
d
n, number of independent experiments.
e
−MLT and +MLT, data obtained prior to or after, respectively, melittin addition.