Table 2.
Fusion of homologous and non-homologous cells: mean ± standard deviation.
| Cell sample | Number of cells (× 106) | Bi-tri nucleated cells (%) | Poly nucleated cells (%) | Single-dye polykaryocytes (%) | Dual-dyes polykaryocytes (%) |
|---|---|---|---|---|---|
|
Ly-Ly 1st step |
98 ± 3.4 | 1.3 ± 0.8 | 0.11 ± 0.22 | 1.44 ± 0.24 | 0.35 ± 0.04 |
|
Ly-Ly 2nd step |
17.9 ± 0.6 | 79.61 ± 0.86 | 8.9 ± 5.2 | 9.5 ± 1.2 | 7.73 ± 2.5 |
|
Er-Er 1st step |
1 ± 0.06 | 0.76 ± 0.01 | 1.04 ± 0.8 | 0.74 ± 0.05 | 0.33 ± 0.66 |
|
Er-Er 2nd step |
7.02 ± 0.11 | 3.3 ± 0.5 | 2.9 ± 0.95 | 2.8 ± 1.09 | 1.44 ± 0.87 |
|
Ly-Mo 1st step |
99 ± 0.91 | 0.48 ± 0.05 | 0.24 ± 0.01 | 0.25 ± 0.09 | 0.22 ± 0.03 |
|
Ly-Mo 2nd step |
70.07 ± 1.85 | 2.23 ± 0.02 | 1.75 ± 0.84 | 1.63 ± 0.78 | 0.71 ± 0.06 |
1000 cells were counted in phase contrast microscopy and in fluorescent microscopy using a filter set appropriate for both emission wavelenghts (CMFDA and CMTMR). (Data from 10 experiments. Ly, lymphocyte; Er, erythroblast; Mo, monocyte.). Single-dye polykaryocytes result from the fusion of cells stained by the same fluorescent dye, while dual-dye polykaryocytes result from the fusion of cells stained bt two different fluorescent dyes.