Table 3.
Isolation of lymphocyte-lymphocyte fusion products by velocity sedimentation, mean ± standard deviation.
| Cell isolation by velocity sedimentation | Mononucleated cells (%) | Bi-tri nucleated cells (%) | Polynucleated cells (%) | |
|---|---|---|---|---|
| Fractions | I | 0.44 ± 0.75 | 0.48 ± 0.63 | 98.88 ± 1.01 |
| II | 0.21 ± 0.20 | 5.09 ± 0.87 | 95.97 ± 0.67 | |
| III | 3.81 ± 0.83 | 95.94 ± 0.82 | 0.33 ± 0.72 | |
| IV | 87.06 ± 0.85 | 13.16 ± 0.85 | 0.2 ± 0.11 | |
1000 cells were counted in phase contrast microscopy and in fluorescent microscopy using a filter set appropiate for both emission wavelenghts (CMFDA and CMTMR). (Data from 10 experiments.)