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. 2011 Oct 5;6(10):e24717. doi: 10.1371/journal.pone.0024717

Figure 4. Validation of 4 target genes of miR-886-3p by quantitative RT-PCR.

Figure 4

Transfection of pre-miR-886-3p significantly decreased the mRNA levels of four target genes (CDC6, PIP5K1C, PXN, ZYX) in A) TPC-1 cell line and B) FTC-133 cell line (*p<0.05; ** p<0.01). The Y axis represents the ratio of the specific target gene and GAPDH using 2-ΔΔCt method, and the value of the miR-NC group was set at 1.0 to allow comparison of fold differences among different cell lines and genes.