Figure 6. Bacterial two hybrid studies on in vivo interactions between chlamydial MreB, MraY and MurG.

Protein interactions were quantitatively determined by measuring β-galactosidase activities of three individually selected replicate cotransformants. The β-galactosidase activities are expressed in Miller Units (change in A420/min/ml/OD₆₀₀) as mean values (± standard deviation). Unpaired t-test revealed statistical significance in comparision to empty vector negative control (T25×T18), *: two-tailed p-value≤0.05, *** two-tailed p-value≤0.001).