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. 2011 Oct 5;6(10):e25480. doi: 10.1371/journal.pone.0025480

Figure 1. C-Xyloside induces sulfated GAGs from NHEK in culture media without the involvement of Xylosyltransferase.

Figure 1

Primary keratinocytes were stimulated with PBS (untreated cells) or C-Xyloside (0.3 mM or 1 mM). (A) Cultured media were collected after 24 and 48 hours. Total sulfated GAG was determined by Blyscan assay. (B), Increasing concentrations of C-Xyloside were added to keratinocytes. Culture media was collected after 24 hours and total sulfated GAG was determined by Blyscan assay. (C), CHO-K1 (black bars) and CHO-745 (white bars) cells were cultured in the presence or absence of C-Xyloside (1 mM) for 24 hours and the amount of sulfated GAG in the media was determined by Blyscan assay. Data are representative of three individual experiments, and presented as mean ± SEM (n = 3). N.D., not detected.