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. 2011 Oct 5;6(10):e25480. doi: 10.1371/journal.pone.0025480

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Media were harvested after 24 hours of culture (A) without or (B) with C-Xyloside (1 mM) stimulation. GAG in culture media were digested with chondroitinase ABC and the disaccharides were resolved by anion-exhange HPLC with post-column derivitization and fluorescence detection (see materials and methods.) Values are expressed as molar percentage. Data are representative of three individual experiments. (C), the disaccharides were resolved by Glycan reductive isotope labeling/mass spectrometry (n = 2). Data are presented as mean ± range. D0a0, ΔUA-GalNAc; D0a4, ΔUA-GalNAc4S; D2a0, ΔUA2S-GalNAc; D0a6, ΔUA-GalNAc6S; D2a4, ΔUA2S-GalNAc4S; D2a6, ΔUA2S-GalNAc6S; D0a10, ΔUA-GalNAc4S6S; D2a10, ΔUA2S-GalNAc4S6S. ΔUA = 4, 5-unsaturated uronic acid.