Skip to main content
. 2011 Oct 5;6(10):e25163. doi: 10.1371/journal.pone.0025163

Figure 4. Validation of two classes of genes, which are dependent or independent of endocytosis.

Figure 4

(A,B) Serum-starved C2C12 cells were treated for 2 h with 40 µM dynasore or 0.05% DMSO prior to stimulation with 30 nM BMP-2 for 6 h in medium containing dynasore or DMSO. Total RNA was isolated, cDNA was synthesized and mRNA expression was analyzed by qPCR using specific mouse primers (Table S1). Histograms show mean normalized expression (MNE) with standard error of duplicate measurements relative to the housekeeping gene HPRT. The analysis shown is representative of three independent experiments. (C) C2C12 cells were co-transfected with Id1-Luc or Id2-Luc and RL-TK. Cells were serum-starved and treated with 40 µM dynasore or 0.05% DMSO for 1 h prior to stimulation with 3 nM BMP-2 for 24 h in medium containing dynasore or DMSO. Relative luciferase activity (RLA) of Id1- or Id2-driven luciferase compared to constitutive expression of RL-TK is shown. Results are shown as mean ± s.d. of triplicate measurements, representative of three independent experiments.