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. 2011 Oct 5;6(10):e25730. doi: 10.1371/journal.pone.0025730

Figure 3. Western blot analysis of GFP protein in ago6 mutants.

Figure 3

Accumulation of GFP protein was observed in all four ago6 mutants as well as nrpe1-10 (defective in the largest subunit of Pol V), demonstrating release of GFP silencing. Control lanes include non-transgenic wild type plants (WT) and transgenic plants containing only the target locus (T) or the target and silencer loci (T+S). All mutants are in a T+S background. Levels of a constitutively expressed tubulin protein are shown as a loading control.