Table 1.

T_h-epitope hotspots in the HIV env protein

^*Mice were immunized with either the 1007 or UG92005 (UG) envs using a prime and double boost regime. 

^† Alignment of epitopes in sequences from the V2, V3, V4, C4 and GP41 regions of the 1007 (Upper) and UG (Lower) env sequences. The shaded residues identify potential sites for N-linked glycosylation. The black boxes show the precise location of the epitopes. 

^‡ The spectrum of Vβ usage is shown for each hybridoma set, with ND designating TCRs that could not be identified with the available panel of mAbs. 

^§ The bracketed figures give the number of distinct clonotypes distinguished based on differences in peptide specificity, differences in TCR usage, or derivation from different mice. Positive IL-2 responses indicating peptide binding activity were approximately 1,280 units/ml, with backgrounds of 0–20 units/ml. 

^¶∥** Identify the Vα chains that could be characterized with the available mAbs to Vα2 (¶), Vα8 (∥), and Vα11 (**).