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. 2011 Oct 5;6(10):e25822. doi: 10.1371/journal.pone.0025822

Figure 7. H342 downregulates SNIP1-stimulated gene expression of c-Myc target genes that are associated with mitochondrial function in H2373 MPM cells.

Figure 7

A, Four gene products of the real-time RT-PCR were visualized after separation on an agarose gel. B, Comparing differences of PCR cycles of four genes. C, Schematic diagram of H342-induced mitochondria dysfunction through SNIP1 and COX19. In untreated H2373 MPM cells, C-terminus of nuclear protein SNIP1 interacts with the N-terminal c-Myc, resulting in enhanced transcriptional activation of c-Myc-dependent genes [30]. In addition, COX19 participates in the biogenesis mitochondrial respiratory chain complexes [35]. H342 rapidly attenuates gene expression of SNIP1 and consequently causes downregulation of c-Myc target genes such as TFAM, resulting in downregulation of gene expression of mitochondria such as COX1 [34]. COX19 downregulation fail to organize cytochrome c oxidase. Overall, these alterations of gene expression induced by H342 may lead to mitochondrial dysfunction, for good reason-H342-induced apoptosis is a mitochondria-mediated apoptosis.