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. 2011 Oct 5;6(10):e25821. doi: 10.1371/journal.pone.0025821

Table 2. Distribution of S. cerevisiae genes in tetrads from the NBRC 1948-CBS 7001 cross.

Markers Methodology CBS NBRC NBCB-2 NBCB-6 CBS NRRL
7001 1948 2a 2b 2c 2d 6a 6b 6c 6d 380T Y-1551
Contig cB Hybridization − cB cB − − cB - cB − cB cB cB
Contig cA Hybridization − cA cA − cA − − − cA cA cA cA
Contig cC Hybridization − cC − cC cC − cC − − cC cC cC
SuMEL PCR or Seq.cing + − − + + − − + − + + +
HO Sequencing UVA LG UVA UVA LG LG UVA LG UVA LG LG LG
ScBIO2 PCR − + + − − + − + − + + +
ScMAL31 specific fragment (a) PCR − + + − − + − + − + + +
MTY1 specific fragment (b) PCR − + + + + − + − + + + +
ScMAL33 PCR − + + + + + + + − + + +
ScBIO2-ScIMA1 PCR − + + − − + − + − + + +
ScIMA1-ScMAL33 PCR − + + + + + + + *** + + +
ScMAL33-(MTY1-ScMAL31) PCR - + + + + + + + − + + +
(MTY1-ScMAL31)-ScMAL32 PCR − + + + + + + + + + + +
ScMAL32-ScCOS2 PCR − + + + + + + + + + + +
ScCOS2-ScSUC4 PCR − + + + + + + + + + + +
ScSUC4-ScRTM1 PCR − + + − (c) − (c) + + + + + + +
Contig cE PCR − + − E − E E − E − + +
NTS2 patterns PCR-RFLP UVAR CARB UVAR CARB CARB UVAR UVAR UVAR CARB CARB CARB CARB
PMA1 PCR-RFLP UVA LG UVA UVA LG LG UVA LG UVA LG UVA LG
MAL locus Sequencing − MAL31/MTY1 ND MTY1 ND MAL31 MTY1 MAL31 MTY1 ND MAL31/MTY1 MAL31/MTY1
Melibiose fermentation + + − + + − − + − + − +
Maltotriose fermentation − + + + + − + − + + + +
Maltose fermentation + + + delay delay + − + delay + + +
Sporulation + + + + + + − + + + + −

(a) Primers MAL31yF (5′TGAGTGGTTTTAGCGTATTC)/MAL31SpR1 (5′ CAAAACTGTAACTACAATTTGG).

(b) Primers MAL31yF ((5′TGAGTGGTTTTAGCGTATTC)/MTYSpR2 (5′CAATAGGAACCTTCTGAG).

(c) RTM amplifiable but SUC4 not amplifiable.

***

The junction between the uvarum part and the cerevisiae part occurred within this fragment for contig cA and gave rise to a PCR fragment shorter than expected. LG: sequence with 93–94% identity with S. uvarum (UVA) sequences.