Table 3. Phylogenetic affiliation of selected 16S bacterial sequences.
Closest relatives (Silva 104) | Number of neighbours >95% in EMBL database | ||||||
DGGE band | similarity (%) | Accession number | Taxonomy | with uranium | with iron | ||
B55_U07_18 | 94.7 | HQ114173 | Acidobacteria | DA023 | uncultured bacterium | - | - |
B55_U09_02 | 99.5 | EU937874 | Acidobacteria | DA023 | uncultured bacterium | 17 | 4 |
B55_U07_12 | 99.5 | HM062339 | Acidobacteria | DA023 | uncultured bacterium | - | - |
B55_U09_13; 20 | 92.3 | EU297423 | Acidobacteria | DA023 | uncultured bacterium | - | - |
B5_U07_03 | 91.1 | FJ004730 | Acidobacteria | DA023 | uncultured bacterium | - | - |
B38_U07_13 | 98.5 | HM061965 | Acidobacteria | DA023 | uncultured bacterium | - | - |
B55_U07_13; B4_U09_03 | 98.9-98.7 | FJ466151 | Acidobacteria | DA052 | uncultured bacterium | 2 | - |
B55_U07_02; 04; 07; 08; 11; 14 | 99.8-99.1 | EU335357 | Acidobacteria | DA052 | uncultured bacterium | 6 | 2 |
B55_U09_07; 10; 11 | 99.6-99.1 | EU335357 | Acidobacteria | DA052 | uncultured bacterium | 6 | 2 |
B55_U06_04; 07 | 99.6 | EU335357 | Acidobacteria | DA052 | uncultured bacterium | 6 | 2 |
B55_U09_01 | 98.3 | FJ004659 | Acidobacteria | DA052 | uncultured bacterium | - | - |
B55_U07_01 | 99.1 | DQ451504 | Acidobacteria | DA052 | uncultured bacterium | 5 | 2 |
B55_U07_16 | 98.9 | EU680444 | Acidobacteria | DA052 | uncultured bacterium | 7 | 2 |
B55_U07_19 | 99.6 | EF018280 | Acidobacteria | DA052 | uncultured bacterium | 6 | 2 |
B4_U09_02; 16 | 99.1-98.2 | GQ342323 | Acidobacteria | Holophagaceae/Geothrix | uncultured bacterium | 8 | 4 |
B14_U07_16 | 98.7 | GQ342323 | Acidobacteria | Holophagaceae/Geothrix | uncultured bacterium | 8 | 6 |
B14_U07_3a | 97.5 | FR667839 | Betaproteobacteria | Gallionellaceae/Sideroxydans | uncultured bacterium | - | 6 |
B4_U07_E11 | 98.9 | AJ582038 | Betaproteobacteria | Gallionellaceae/Sideroxydans | uncultured bacterium | 1 | 74 |
B4_U09_08 | 99.8 | AJ582038 | Betaproteobacteria | Gallionellaceae/Sideroxydans | uncultured bacterium | 2 | 76 |
B38_U07_05 | 94 | AB240323 | Betaproteobacteria | Gallionellaceae | uncultured bacterium | - | - |
B14_U07_04 | 96.2 | AB240324 | Betaproteobacteria | Gallionellaceae | uncultured bacterium | - | 17 |
B4_U07_E16 | 93.3 | DQ450774 | Betaproteobacteria | Gallionellaceae | uncultured bacterium | - | - |
B5_U07_2f | 99.3 | DQ778036 | Gammaproteobacteria | Pseudomonadaceae | Pseudomonas sp.AD21 | 71 | 82 |
B5_U07_18 | 100 | GU569131 | Gammaproteobacteria | Pseudomonadaceae | uncultured Pseudo. sp. | 63 | 81 |
B5_U07_12 | 91.3 | GU270815 | Deltaproteobacteria | Geobacteraceae | uncultured bacterium | - | - |
B14_U07_05 | 91.3 | AY607189 | Deltaproteobacteria | Geobacteraceae | uncultured Geobacter | - | - |
B4_U09_13 | 97.8 | FR667779 | Deltaproteobacteria | Geobacteraceae | uncultured bacterium | 9 | 17 |
B5_U07_09 | 98.7 | CP001124 | Deltaproteobacteria | Geobacteraceae | Geobacter bemidjiensis | 10 | 6 |
The sequences derived from DGGE bands characterizing uranium-rich soil samples from Villard. The number of close relatives (having >95% sequence similarity), detected in uranium-contaminated or iron-rich environments is indicated in the two last columns.