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. 2011 Oct 5;6(10):e25924. doi: 10.1371/journal.pone.0025924

Figure 5. CADY:siRNA complexes induce cellular influx of calcium.

Figure 5

HeLa cells were incubated with 5 µM FURA-2AM followed by washes in HEPES-Krebs-Ringer (HKR) buffer (A–C,E) or HKR buffer without calcium (D,E). The ratio of 340(Ca-bound FURA-2AM)/380(FURA-2AM) nm excitatory wavelengths was recorded upon addition of 20 nM siRNA complexed to CADY at a 1∶20 molar ratio. HeLa cells were transfected with 80 nM FITC labeled siRNA complexed to CADY at a 1∶20 molar ratio in the presence (F) or absence (G) of calcium. After 90 mins, cells were washed, trypsinized and analysed by FACS.