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. 2011 Oct 5;6(10):e25973. doi: 10.1371/journal.pone.0025973

Figure 6. Reduced H3K4 methylation by Set1/MLL depletion does not enhance DCC mislocalization in htz-1(tm2469).

Figure 6

DPY-27 (green) and X-Paint FISH (red) colocalization analysis upon Set1/MLL depletion in htz-1(tm2469)m+z- mutant animals indicates that reduced H3K4 methylation does not further disrupt DCC localization. In all panels DAPI is shown in grayscale and scale bar equals 5 µm. B. Quantification of colocalization by Pearson's correlation coefficient R indicating the degree of overlap between the DCC and X-Paint masks. R measurements were binned every 0.1 from 0 to 1 (where 0 indicates no detectable overlap and 1 indicates perfect colocalization) and percent of total sample per bin is shown. See Figure S7 for statistical analysis.