Fig. 3.

Transcription initiation sites and putative promoters of the ectoine synthesis genes ectABC. Nucleotide sequences of the ectA (A) and ectC (B) promoter regions. Arrows indicate the transcription initiation sites (+ 1), which were mapped by RACE-PCR. The −35 and −10 sequences of the σ⁷⁰ and σ³⁸ promoters upstream of ectA, and the −24 and −12 sequences of the σ⁵⁴ promoter upstream of ectC are written in bold. (B) An 18 bp sequence GCCCGCTGACCATT is located at −111 to −128 bp upstream of the ectC transcription initiation site that resembles the consensus sequence of σ⁵⁴ promoters that are controlled by FleQ (not displayed).