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. 2001 Apr 3;98(8):4593–4598. doi: 10.1073/pnas.071050798

Figure 3.

Figure 3

Imm-H induces apoptosis in human T cell lines but not in the BL2 B cell leukemia line. In A, flow cytometry analysis of the malignant CCRF-CEM T cell leukemia line gated for annexin+/pI− incubated for 36 h with 10 μM Imm-H and 20 μM dGuo. The percent of cells within each quadrant is indicated. The lower right quadrant corresponds to cells undergoing apoptosis. In B, the gated percent of apoptotic cells from flow cytometry for CCRF-CEM and BL2 cells incubated for 36 h with 10 μM Imm-H, 20 μM dGuo, and 20 μM dCyd, as indicated (Imm-H, dGuo, dCyd). Only CCRF-CEM T cell line undergoes apoptosis in the presence of dGuo and Imm-H (Imm-H, dGuo). Culture conditions that rescue CCRF-CEM from growth inhibition by dGuo and Imm-H do not induce apoptosis (Imm-H and Imm-H, dCyd). Similar results were obtained with the MOLT-4 leukemia cell line (data not shown). BL2 are resistant to apoptosis after exposure to Imm-H.