Table 2.
Effect of Imm-H on dGTP levels in CCRF–CEM cells
Growth condition* | GTP | dGTP |
---|---|---|
(nmol/5 × 106 cells†) | ||
Control‡ | 1.86 | ND§ |
dGuo | 2.17 | ND¶ |
dGuo + Imm-H | 0.16 | 1.53 |
Cells were cultured for 24 hr in the presence of 10 μM dGuo with or without 10 μM Imm-H.
Nucleotide content was determined from GTP and dGTP standards analyzed by using HPLC chromatography. Experiments were performed in duplicate with errors estimated at ±10%.
Result from Bantia et al. (22).
¶ Not detectable (limit of detection = 0.2 and 0.08, respectively).