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. 1984 May 11;12(9):3821–3827. doi: 10.1093/nar/12.9.3821

Elimination of mRNA splicing by a point mutation outside the conserved GU at 5' splice sites.

C Montell, A J Berk
PMCID: PMC318792  PMID: 6587322

Abstract

Nearly all mRNA introns begin with the dinucleotide GU. Mutations in either of these virtually invariant bases have been found to inactivate the corresponding 5' splice site. Until now single base changes in neighboring bases have not been found to completely inactivate a 5' splice site. Here we show that a single A----U transversion in the third position of the adenovirus 2 E1A 13S mRNA intron does prevent RNA splicing at the corresponding 5' splice site.

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Selected References

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