Figure 6. JQ1 induces cell cycle arrest and cellular senescence in MM cells.
(A-B) Flow cytometric evaluation of propidium iodide (PI) staining for cell cycle analysis (panel A) and detection of Annexin V-positive apoptotic cells (panel B) in JQ1-treated MM.1S cells (0 - 48hrs, 500 nM).
(C) Enrichment of senescence-associated genes among JQ1-suppressed genes in MM.1S cells.
(D) Induction of cellular senescence in JQ1 treated MM.1S cells (500 nM, 72 h), as detected by ß-galactosidase staining.