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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Future Virol. 2011 Sep;6(9):1045–1058. doi: 10.2217/fvl.11.76

Figure 1. Expression of nectin-like molecule-5 in glioblastoma.

Figure 1

(A) A panel of six primary-explant glioblastoma (GBM) cultures were infected with PVSRIPO and images of the infected cultures were acquired at the indicated time points of hours postinfection. At a total of 12 h postinfection, all cells were lysed in all samples. The cytopathic effects of PVSRIPO in laboratory glioma cell lines (e.g., HTB14 or DU54) have been reported previously (Figure 5D) [26,27]. (B) Quantification of PVSRIPO levels in infected cultures indicates proficient propagation of the virus in infected primary-explant GBM cells. (C) Immunoblot analyses of nectin-like molecule-5 in homogenates obtained from primary explant cells (C) or in homogenates from GBM tissues directly (T). HTB14 and DU54 are established laboratory glioma cell lines. Abundant nectin-like molecule-5 expression in primary-explant GBM cultures equals the observed levels in GBM patient tissues or in established laboratory cell lines.

C: Cell; T: Tissue.

Reproduced from [27] by permission of Oxford University Press.