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. 2011 Oct;18(10):654–665. doi: 10.1101/lm.2224411

Figure 4.

Figure 4.

Propanol-specific associative learning. (A) Wild-type worms were conditioned 10 times simultaneously with 1-propanol and HCl by spaced (with a 10-min ITI) or massed training. Immediately after the training, worms were assayed for their ability of chemotaxis to 5% 1-propanol, 0.01% benzaldehyde, 0.001% isoamyl alcohol, or 0.001% diacetyl spotted on the edge of chemotaxis agar plates. Note that chemotactic behaviors of the trained worms to benzaldehyde, isoamyl alcohol, and diacetyl were not affected by the training. Data are means ± SEM (n = 9 assays). Asterisks indicate statistically significant differences (**P <0.01) determined by one-way ANOVA with Bonferroni/Dunn test, in comparison to the CI of naive worms (before training). (B) Associative learning of 1-propanol with HCl was specific for 1-propanol. LI values were calculated from the data shown in A. Data are means ± SEM (n = 9 assays). Asterisks indicate statistically significant differences (**P <0.01) determined by one-way ANOVA with Bonferroni/Dunn test, in comparison to the LI of worms assayed with 1-propanol as a stimulus.