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. 2001 Mar 27;98(8):4599–4604. doi: 10.1073/pnas.081021898

Figure 5.

Figure 5

MKK4 gene disruption does not affect cytokine gene expression in ESMCs. MKK4+/+ or MKK4−/− ESMCs were passively sensitized with anti-OVA IgE and stimulated with OVA for 0, 30, 60, 120, and 180 min. Cells were harvested, and total RNA was isolated. Fifteen micrograms of total RNA was used in the RNase protection assay. The RNA probes for IL-4, IL-5, TNF-α, IL-13, IL-15, IL-9, IL-2, IL-6, and IFN-γ were used, in addition to L32 and glyceraldehyde-3-phosphate dehydrogenase as controls. Representative autoradiographs from two independent experiments are shown.