Fig. 3. Expression of TLR-4 on skin DC at 7 day after burn injury. Total skin cells were isolated from burn patients and healthy subjects and stimulated in the presence or absence of LPS. After 24 h, the expression of TLR-4 on DCs was determined by means of fluorescence intensity. DCs from healthy subjects and from non-burned skin expressed higher basal levels (A) of TLR-4 than those from burned skin (p < 0.001). After LPS stimulation (B) these levels further increased, albeit to a different extent. Histograms represent the mean ± SD in each group.