FIG 2 .

Distribution of predivisional, early divisional, and late-divisional cells with SecA-L-FLAG³ (A) and HtrA-L-FLAG³ (B) localized at equators, division septa, or hemispheres between septa and future equators, as indicated. Cells were grown exponentially (log) or to the transition to stationary phase, and IFM was performed as described in Materials and Methods. (A) cls⁺ parent strain IU4472 (D39 ∆cps rpsL1 secA-L-FLAG³), ∆cls::P_c-erm mutant IU5093, and complemented ∆cls mutant IU5138 (Δcls::P_c-erm//CEP::cls⁺, where “//” indicates a separate ectopic location in the chromosome). (B) cls⁺ parent strain IU4468 (D39 ∆cps rpsL1 htrA-L-FLAG³), ∆cls::P_c-erm mutant IU5089, and complemented ∆cls mutant IU5136 (∆cls::P_c-erm/CEP::cls⁺). See the text for additional details.