FIG 3 .

SecA-L-FLAG³ (A) and HtrA-L-FLAG³ (B) do not localize in cells during the transition from exponential to stationary phase in BHI broth. Strains IU4472 (Fig. 1) and IU4468 (Fig. 4) were grown to transition phase, and DAPI staining and IFM of cells were performed as described in Materials and Methods. Representative cells are shown with the same column arrangement as in Fig. 1. The experiment was performed more than 2 times independently. Multiple fields were observed in each experiment, with similar results. Scale bar = 1 µm.