FIG 4 .

HtrA-L-FLAG³ localizes to equators and septa of exponentially growing S. pneumoniae cells. Strain IU4468 (D39 ∆cps rpsL1 htrA-L-FLAG³) was grown to mid-exponential phase in BHI broth, and DAPI staining and IFM of cells were performed as described in Materials and Methods. Representative images are shown with the same row and column arrangements as in Fig. 1. A schematic summary at right shows the HtrA_Spn distribution pattern in >90% of the cell population (Fig. 2). The experiment was performed more than 3 times independently. Multiple fields were observed in each experiment, with similar results. Scale bar = 1 µm.