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. Author manuscript; available in PMC: 2012 Sep 21.
Published in final edited form as: J Am Chem Soc. 2011 Aug 26;133(37):14704–14709. doi: 10.1021/ja204516p

Figure 2.

Figure 2

Bioconjugation of HOPO-maleimide 6a to N87C MS2 and mass spectral characterization of the MS2-conjugates (See Supporting Information for additional spectra). (a) Conditions: 10 mM phosphate, TRIS buffer pH 8, 6a (20 eq), 3h. (b) ESI-MS reconstruction of MS2 N87C monomers after capsid dissociation. Expected mass: [M+H+] = 13719 m/z. (c) ESI-reconstruct of linear linker protein conjugate 7a, showing virtually complete conversion (corresponding to 180 copies per capsid). Expected mass of HOPO-linear-MS2: [M+H+] = 14469 m/z. All mass values agreed to within 0.03% of those expected. Full charge ladders appear in Figure S1 of the Supporting Information.